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ABG interpretation in five steps

A systematic ABG walkthrough — pH, primary disorder, compensation, anion gap and Winter's formula — so mixed disorders are reasoned out, not guessed.

6 min read · updated 2026-08-24

Read every ABG the same way

Never eyeball an ABG. Run the same five steps in order and mixed disorders reveal themselves. Normal values to anchor: pH 7.35–7.45, PaCO₂ 35–45 mmHg, HCO₃⁻ 22–26 mmol/L.

Step 1 — Look at the pH

  • pH < 7.35 → acidaemia; pH > 7.45 → alkalaemia.
  • A "normal" pH with abnormal PaCO₂ and HCO₃⁻ means a compensated or mixed disorder — do not stop here.

Step 2 — Identify the primary disorder

Ask which value — PaCO₂ or HCO₃⁻ — moved in the same direction as the pH change.

  • HCO₃⁻ tracks the pH → metabolic. PaCO₂ moves opposite to pH → respiratory.
Primary disorderpHPrimary changeCompensation (direction)
Metabolic acidosis↓ HCO₃⁻↓ PaCO₂ (hyperventilation)
Metabolic alkalosis↑ HCO₃⁻↑ PaCO₂ (hypoventilation)
Respiratory acidosis↑ PaCO₂↑ HCO₃⁻ (renal retention)
Respiratory alkalosis↓ PaCO₂↓ HCO₃⁻ (renal excretion)

Step 3 — Check the compensation is appropriate

The body never overcompensates — if the "compensation" overshoots the normal range for pH, a second primary disorder is present.

Primary disorderExpected compensation
Metabolic acidosisWinter's: PaCO₂ = 1.5 × HCO₃⁻ + 8 (± 2)
Metabolic alkalosisPaCO₂ rises ~0.7 mmHg per 1 mmol/L rise in HCO₃⁻
Acute respiratory acidosisHCO₃⁻ ↑ 1 per 10 mmHg ↑ PaCO₂
Chronic respiratory acidosisHCO₃⁻ ↑ 4 (3.5) per 10 mmHg ↑ PaCO₂
Acute respiratory alkalosisHCO₃⁻ ↓ 2 per 10 mmHg ↓ PaCO₂
Chronic respiratory alkalosisHCO₃⁻ ↓ 4–5 per 10 mmHg ↓ PaCO₂

Winter's trap: if measured PaCO₂ is higher than Winter's predicts, there is a concurrent respiratory acidosis; if lower, a concurrent respiratory alkalosis.

Step 4 — Anion gap (every metabolic acidosis)

AG = Na⁺ − (Cl⁻ + HCO₃⁻), normal 8–12 mmol/L. Correct for albumin: add 2.5 to the gap per 1 g/dL fall below 4.

  • High-AG acidosis (MUDPILES): Methanol, Uraemia, DKA, Propylene glycol, Iron/INH, Lactic acidosis, Ethylene glycol, Salicylates.
  • Normal-AG (hyperchloraemic): diarrhoea, renal tubular acidosis, acetazolamide.

Step 5 — Delta ratio (in a high-AG acidosis)

Δ ratio = (AG − 12) / (24 − HCO₃⁻). <1 suggests a coexisting normal-AG acidosis; >2 suggests a coexisting metabolic alkalosis or chronic respiratory acidosis.

Pearl: A high anion gap is the one number you must compute even when the pH is normal — it exposes a hidden organic acidosis that compensation has masked.

Anchor

pH → primary → is compensation appropriate? → anion gap → delta. The overshoot that "compensation" can never do is your signal for a second disorder.

Sources

  • Harrison's Principles of Internal Medicine, 21e
  • Ganong's Review of Medical Physiology, 26e